Composite

Part:BBa_I716166:Design

Designed by: Kristin Doan   Group: iGEM07_Berkeley_UC   (2007-08-08)


[RBS][hemA from R.cap][RBS][hemD]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1783
    Illegal PstI site found at 2307
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal NheI site found at 52
    Illegal PstI site found at 1783
    Illegal PstI site found at 2307
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2292
    Illegal EcoRI site found at 1
    Illegal BglII site found at 59
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1783
    Illegal PstI site found at 2307
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1783
    Illegal PstI site found at 2307
    Illegal NgoMIV site found at 927
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3394


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

Composite part of hemA from R. capsulatus + hemB
Digest pBca9145-I716163		        (Bg1II/XhoI, 2112+1010, S)
Sub into pBca9145-I716161		(BamH1/XhoI, 3344+9, L)
Product is pBca9145-I716166


Source

Composite part

References